Genetic bases of the temperature-sensitive phenotype of a master donor virus used in live attenuated influenza vaccines: A/Leningrad/134/17/57 (H2N2).
Identifieur interne : 000C60 ( Main/Exploration ); précédent : 000C59; suivant : 000C61Genetic bases of the temperature-sensitive phenotype of a master donor virus used in live attenuated influenza vaccines: A/Leningrad/134/17/57 (H2N2).
Auteurs : Irina Isakova-Sivak [États-Unis] ; Li-Mei Chen ; Yumiko Matsuoka ; J Theo M. Voeten ; Irina Kiseleva ; Jacco G M. Heldens ; Han Van Den Bosch ; Alexander Klimov ; Larisa Rudenko ; Nancy J. Cox ; Ruben O. DonisSource :
- Virology [ 1096-0341 ] ; 2011.
Descripteurs français
- KwdFr :
- Génotype, Humains, Mutation faux-sens, Méthode des plages virales, Phénotype, Plasmides, Protéines virales (génétique), Protéines virales (métabolisme), RNA replicase (génétique), RNA replicase (métabolisme), Russie, Sous-type H2N2 du virus de la grippe A (croissance et développement), Sous-type H2N2 du virus de la grippe A (génétique), Température, Vaccins antigrippaux (génétique), Vaccins atténués (génétique), Vecteurs génétiques.
- MESH :
- croissance et développement : Sous-type H2N2 du virus de la grippe A.
- génétique : Protéines virales, RNA replicase, Sous-type H2N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués.
- métabolisme : Protéines virales, RNA replicase.
- Génotype, Humains, Mutation faux-sens, Méthode des plages virales, Phénotype, Plasmides, Russie, Température, Vecteurs génétiques.
English descriptors
- KwdEn :
- Genetic Vectors, Genotype, Humans, Influenza A Virus, H2N2 Subtype (genetics), Influenza A Virus, H2N2 Subtype (growth & development), Influenza Vaccines (genetics), Mutation, Missense, Phenotype, Plasmids, RNA Replicase (genetics), RNA Replicase (metabolism), Russia, Temperature, Vaccines, Attenuated (genetics), Viral Plaque Assay, Viral Proteins (genetics), Viral Proteins (metabolism).
- MESH :
- chemical , genetics : Influenza Vaccines, RNA Replicase, Vaccines, Attenuated, Viral Proteins.
- chemical , metabolism : Viral Proteins.
- geographic : Russia.
- genetics : Influenza A Virus, H2N2 Subtype.
- growth & development : Influenza A Virus, H2N2 Subtype.
- chemical , metabolism : RNA Replicase.
- Genetic Vectors, Genotype, Humans, Mutation, Missense, Phenotype, Plasmids, Temperature, Viral Plaque Assay.
Abstract
Trivalent live attenuated influenza vaccines whose type A components are based on cold-adapted A/Leningrad/134/17/57 (H2N2) (caLen17) master donor virus (MDV) have been successfully used in Russia for decades to control influenza. The vaccine virus comprises hemagglutinin and neuraminidase genes from the circulating viruses and the remaining six genes from the MDV. The latter confer temperature-sensitive (ts) and attenuated (att) phenotypes. The ts phenotype of the vaccine virus is a critical biological determinant of attenuation of virulence. We developed a plasmid-based reverse genetics system for MDV caLen17 to study the genetic basis of its ts phenotype. Mutations in the polymerase proteins PB1 and PB2 played a crucial role in the ts phenotype of MDV caLen17. In addition, we show that caLen17-specific ts mutations could impart the ts phenotype to the divergent PR8 virus, suggesting the feasibility of transferring the ts phenotype to new viruses of interest for vaccine development.
DOI: 10.1016/j.virol.2011.01.004
PubMed: 21315402
Affiliations:
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Cox</name></author><author><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O" last="Donis">Ruben O. 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The vaccine virus comprises hemagglutinin and neuraminidase genes from the circulating viruses and the remaining six genes from the MDV. The latter confer temperature-sensitive (ts) and attenuated (att) phenotypes. The ts phenotype of the vaccine virus is a critical biological determinant of attenuation of virulence. We developed a plasmid-based reverse genetics system for MDV caLen17 to study the genetic basis of its ts phenotype. Mutations in the polymerase proteins PB1 and PB2 played a crucial role in the ts phenotype of MDV caLen17. In addition, we show that caLen17-specific ts mutations could impart the ts phenotype to the divergent PR8 virus, suggesting the feasibility of transferring the ts phenotype to new viruses of interest for vaccine development.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Chen, Li Mei" sort="Chen, Li Mei" uniqKey="Chen L" first="Li-Mei" last="Chen">Li-Mei Chen</name><name sortKey="Cox, Nancy J" sort="Cox, Nancy J" uniqKey="Cox N" first="Nancy J" last="Cox">Nancy J. Cox</name><name sortKey="Den Bosch, Han Van" sort="Den Bosch, Han Van" uniqKey="Den Bosch H" first="Han Van" last="Den Bosch">Han Van Den Bosch</name><name sortKey="Donis, Ruben O" sort="Donis, Ruben O" uniqKey="Donis R" first="Ruben O" last="Donis">Ruben O. Donis</name><name sortKey="Heldens, Jacco G M" sort="Heldens, Jacco G M" uniqKey="Heldens J" first="Jacco G M" last="Heldens">Jacco G M. Heldens</name><name sortKey="Kiseleva, Irina" sort="Kiseleva, Irina" uniqKey="Kiseleva I" first="Irina" last="Kiseleva">Irina Kiseleva</name><name sortKey="Klimov, Alexander" sort="Klimov, Alexander" uniqKey="Klimov A" first="Alexander" last="Klimov">Alexander Klimov</name><name sortKey="Matsuoka, Yumiko" sort="Matsuoka, Yumiko" uniqKey="Matsuoka Y" first="Yumiko" last="Matsuoka">Yumiko Matsuoka</name><name sortKey="Rudenko, Larisa" sort="Rudenko, Larisa" uniqKey="Rudenko L" first="Larisa" last="Rudenko">Larisa Rudenko</name><name sortKey="Voeten, J Theo M" sort="Voeten, J Theo M" uniqKey="Voeten J" first="J Theo M" last="Voeten">J Theo M. Voeten</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Isakova Sivak, Irina" sort="Isakova Sivak, Irina" uniqKey="Isakova Sivak I" first="Irina" last="Isakova-Sivak">Irina Isakova-Sivak</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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